How this Food Allergy Test works.
This test requires you to bring your package to a nurse or doctor who can take a Venous blood sample for you. You then post this off in the special pack provided to BIO Lab UK who will send you back a report within 10 days with your allergy test results.
Who should take this test.
If you are one of the 2% of people who suffer from what you think is severe allergic symtoms to a food or allergen this test is for you. If you would like to contact Mary in advance if you are not sure if you should take this test or a Food Intolerance Test you can read more just click here
Allergen-specific IgE Testing in Cork for Food and Inhalent Allergies
Adverse reactions to food and to inhaled substances can arise by a variety of mechanisms and these may be immune- or non-immune mediated. The usefulness of measuring IgE antibodies in Type 1 hypersensitivity reactions is now well established as a means to identify the allergens responsible for this immune-mediated reaction , which can be triggered by very small amounts of antigen.
Immune-mediated hypersensitivity reactions were classified by Coombes and Gell  into four types:
- Type 1 (immediate hypersensitivity) reactions are mediated by IgE antibodies; examples are hay fever and nut allergies.
- Type 2 (antibody-dependent, cytotoxic) reactions are mediated by IgG and IgM antibodies, as well as complement; for example Goodpasture’s disease.
- Type 3 (immune complex) reactions are mediated by IgG and complement; for example SLE (systemic lupus erythrematosis).
- Type 4 (delayed hypersensitivity) reactions are mediated by T cells; for example the contact dermatitis that characterises nickel hypersensitivity.
Over one third of the UK population has been diagnosed with asthma, eczema or hay fever . The consensus is that this is largely Type 1 hypersensitivity. There has also been a continuous growth in anaphylactic allergic events presenting in Accident and Emergency Departments. In particular the prevalence of Type 1 peanut allergy is increasing .
Clinical manifestations of Type 1 (immediate hypersensitivity) diseases are caused by the release of proinflammatory mediators (such as histamine, leukotrienes and prostaglandins) from IgE-sensitized effector cells (mast cells and basophils) when cell-bound IgE antibodies interact with the allergen. Thus in vitro serum testing for IgE antibodies provides an indication of the immune response to the allergen(s) that may be associated with the disease.
While the definitive test for proving a suspected food allergy is a food challenge, this carries a significant risk to the patient and is, in most cases, impractical, so confirmation of a positive result of an allergenspecific IgE test can be made by skin prick testing (which is widely available). In most cases total IgE measurement is of relatively little value, since approximately one third of atopic adults have a total serum IgE of less than 120 kU/L, which is within the reference range for non-atopic individuals (as determined by most laboratories). However, for very high levels of total IgE, allergic disease is more likely . There is also evidence to suggest that in infants a raised total serum IgE is predictive allergy in later life 
Antigen-specific IgE tests
Testing for IgE antibodies may be useful to establish the diagnosis of an allergic disease and to define the allergens responsible for eliciting the signs and symptoms observed. Testing also may be useful to identify the allergens responsible for an anaphylactic episode, or to confirm sensitization to particular allergens prior to beginning immunotherapy.
The presence of an IgE antibody in the serum indicates that the patient has been sensitized to a food or inhalant allergen, i.e. has generated an immune response resulting in the production of an antibody.
There are no international standards for specific IgE assays, which are instead calibrated using the WHO reference preparation for total serum IgE (75/702) . Biolab measures allergen-specific IgE by luminometry, using Optigen® reagents for the determination.
What does it test for?
There are two panels available:
- an inhalant panel for detecting antibodies against English Plantain, Mugwort, Pellitory, Lamb's Quarters, Ragweed (short), Olive, Oak (white), Beech (American), white birch, v Ash, Cypress (Italian), Alder (black), London Plane, Alternaria, Candida, Aspergillus, Latex, Cockroach mix, Kentucky Blue Grass, Timothy Grass, Orchard Grass (cockfoot), Bermuda Grass, Wheat pollen, Rye (cultivated), Dog, Horse, Cat, Blomia (tropicalis), Dermatophagoides farinae and D. pteronyssinus (house dust mites).
- a food panel for detecting antibodies against crab, codfish, carrot, apple, potato, tomato, celery, peach, walnut, peanut, hazelnut, almond, soyabean, rye, wheat, sesame, casein, egg white and egg yolk.
Interpretation of results
The level in serum of IgE antibody to each allergen is expressed in luminometry units (LU), which can be equated to a class score or kU/L. These are split into allergy level classes 0, 0/1, 1, 2, 3, 4. 0 being no antibodies detected and 4 being very high levels of antibodies detected.
Detection of IgE antibodies in serum at Class 1 level or greater indicates an increased likelihood of allergic disease as opposed to other aetiologies and also defines the allergens that may be responsible for eliciting the signs and symptoms.
Testing for IgE antibodies is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists, or in patients in whom the medical management does not depend upon identification of a specific allergen .
Some individuals with clinically insignificant sensitivity to allergens may have measurable levels of IgE antibodies in serum (Class 0/1), and results must be interpreted in the clinical context.
False-positive results for IgE antibodies can occur in patients with markedly elevated total serum IgE (>2500 kU/L) due to nonspecific binding to allergen solid phases. There are also “panallergens”, for example birch pollen, which have similar epitopes in proteins from different species and are thus a potential cause of falsepositive reactions.
Beside each item tested for you will see a score which indicates the level of your allergy to that item, if any. The level in serum of IgE antibody to each allergen is expressed in luminometry units (LU), which can be equated to a class score or kU/L, as shown below:
| 0 - 11|
12 - 26
27 - 65
66 - 142
143 - 242
|0 - 0.34|
0.35 - 0.69
0.70 - 3.49
3.50 - 17.5
|No antibodies detected|
Very low levels of antibodies detected
Low levels of antibodies detected
Moderate levels of antibodies detected
High levels of antibodies detected
Very high levels of antibodies detected
Patient preparation: no special preparation is necessary and the patient can continue taking supplements prior to venipuncture.
Specimen requirements: the following blood specimen is required - 1 clotted SST (serum separator, yellow top) tube. A postal sample kit can be supplied.
Total serum IgE can be measured as a separate request.